Intensity clearly above background was detected for a 35 nM solution (dotted line), corresponding to ∼2 dye molecules per confocal volume (250 nm × 250 nm × 900 nm, x y z).
Furthermore, no background effects were detected for any behavior.
Very low, almost background transcript levels were detected for W109669 (Additional file 6D), suggesting that this gene is most likely involved primarily in defense responses, whereas W100630, W100577 and W108715 are also expressed during plant development.
In any given sample type, transcripts were detected for 10,067 to 17,070 genes (hybridization signal above background threshold); transcripts were detected for 21,939 different genes considering all of the tissues, i.e. 92% of the P. glauca genes represented on the microarray.
We detected six QTLs for yield in the Koshihikari background and four in the Takanari background, but no QTLs for yield common to both backgrounds were detected, and all QTLs had negative effects on yield.
Only background fluorescence was detected for the mismatched OCPs.
No expression above background levels was detected for both promoters in sugar beet roots (Fig. 4C).
As can be seen in Figure 4 C, after the photobleaching, only background signal was detected for mVenus, while a careful comparison of the mTFP1 images already reveals an increased brightness of the mTFP1 molecules.
