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NIL3, NIL12 and NIL3 12 possessing the deepwater response QTL on chromosomes 3, 12 and 3/12 in the T65 genetic background were derived previously (Hattori et al. 2009).
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Myd88−/−, TLR2−/−, Tlr4−/−, mice (129SvJ×C57Bl/6 background) were derived as previously described [2] and backcrossed for six generations with C57Bl/6 mice.
CCR2−/− mice on a C57BL/6J background were derived as described previously (23) and backcrossed to C57BL/6J mice from the Jackson Laboratory (Bar Harbor, ME, USA) for 6 generations.
A large number of D. subobscura isochromosomal lines for the O chromosome in an otherwise homogeneous genetic background were derived from an outbred stock collected at Puerto Montt (Chile; 41° 28' S) in November 1999 as previously indicated [ 38].
Socioeconomic background was derived using the Townsend Index.
Plasmids were derived from previously generated construct library58.
Primary bone-marrow derived macrophages (BMDMs) were derived as previously described [50].
Bone marrow derived mesenchymal stem cells were derived as previously described [25], [44], [45].
Apparent dissociation constants (K d ) were derived as previously described [ 26].
Both the start codon introns and the background set were derived from proteins without signal peptides.
Care dependency and background characteristics were derived from residents' record by the RN.
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