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Male ApcΔ14/+ animals on the C57BL/6 background were crossed with female FHL2−/− mice on the hybrid Black Swiss-129-SV/J background [32], [33].

To generate αB-crystallin/HspB2 deficient mice expressing the transgene, transgenic mice in 129SvEv background were crossed with the CRYAB-/ mice.

The resultant homozygous conditional NPY-Cre-expressing transgenic mice on a mixed C57/BL6-129/SvJ background were crossed with Y2lox/lox mice on the same background.

Prom1 knock-in mice that were backcrossed and maintained on a C57BL/6 genetic background, were crossed with ACTB-FLPe mice [30] to delete the neomycin selection cassette to generate Prom1lacZ,DTA/+ mice.

In order to investigate the timing of the lethality more closely and to examine embryo viability in a pure 129/SvEv background, Ctgf Lo/Lo mice on a 129/SvEv background were crossed with 129.Tg(Ella-Cre) mice.

GarsC201R/+ heterozygous females (n = 14, N1-4 on the C57BL/6 background) were crossed with SOD1G93A males to produce the expected four genetically distinct groups of littermates: wildtype 26 males and 22 females (total 48); SOD1G93A 19 males and 19 females (total 38); GarsC201R/+ 15 males and 21 females (total 36); SOD1G93A;GarsC201R/+ 15 males and 18 females (total 33).

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To map the GNS2 locus, homozygous gns2 npr1, which is in Columbia (Col-0) background, was crossed with the polymorphic ecotype Landsberg erecta (L er).

arc2 (Ler background) was crossed with Col-0 wild-type plants to generate a mapping population of 308 F2 mutants identified based on their pale phenotype and enlarged chloroplasts.

To produce plants that co-expressed GFP VTI13 and yellow fluorescent protein γ-tonoplast intrinsic protein γ-tonoplast a transgenintrinsicxproteing 35S:GFP–VTI13 in a wild-type background was crossed with the 35S:YFP-γTIP transgenic line (Nexpressingl., 2007), and heterozygous F1 seedlings were analysed by confocal microscopy.

For knockdown experiments, w+ ;UAS-fru C -shmiR virgins (CS background) were crossed to GAL4 lines with various genetic backgrounds.

(Top) BAC WT LRRK2 mice on the FVB background strain were crossed with Tau-Responder mice, also on the FVB background strain, for one generation.

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