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Spots of the PLA signal above background were counted using a semiautomated script written in IP-Lab.
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Viruses were counted using BD CellQuest PRO (v 4.0.1), and background counts from SYBR Green stained and latex bead spiked Tris-EDTA controls were subtracted from final viral enumerations.
6) Counting: plasma samples were counted using appropriate standards and blanks for background in a well counter.
The plates were counted using an automated system (AID, Strasbourg, Germany) and background counts obtained in the absence of peptide or VLP were subtracted.
Cells were counted using the ImageJ software.
Live cells were counted using a hemocytometer.
Stained nuclei were counted using ImageJ.
Cells were counted using ImageJ program (NIH).
Cells were counted using a Bürker hemocytometer.
Protoplasts were counted using a hemocytometer.
Nuclear markers were counted using ImageJ.
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