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For the experiments, homozygous AgrpDTR/DTR males (on mixed 129/Sv×C57Bl/6 genetic background) were bred with C57Bl/6 wild-type, female Agrp+/+ mice, such that all the offspring would be AgrpDTR/+ heterozygotes.
Mice carrying the testicular feminization mutation (Tfm) (Jackson Laboratories, Bar Harbor, Maine, USA) on a C57BL/6J-A-Ta<6J> C57BL/6J-A-Ta<6J> C57BL/6J-A-Ta<6J>/− mice and F1 litters were examined.
Transgenic mice expressing Cre-EIIa on a C57BL/6 background were bred with homozygous Cryaa knock-in mice to delete the neor gene.
Female TRAMP mice on a C57BL/6 background were bred with non-transgenic males, and the offspring was weaned at 3 4 weeks of age.
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All mice with 129/Sv genetic background were bred at l'institut du thorax (Nantes, France) and genotyped by polymerase chain reaction (PCR) as previously described [9].
Mice with the CBA/CaH Spir2 locus on a BALB/cByJ background were bred to the N6 generation before intercrossing.
Mice with the BALB/cByJ Spir2 locus on a CBA/CaH background were bred to the N7 generation and were named CBABYJN7-4.
ApoE−/− mice, also on a C57BL/6 background, were bred in house.
Mice on the C57Bl/6J background were bred in specific pathogen free conditions.
Because the homozygous db/db mice are infertile, heterozygous (Lepr db/+ ) mice on a C57Bl/6 J background (Jackson Labs; Bar Harbor, ME) were bred with APP/PS1 mice on a CD-1/129 CD-1/129nd (obackgroundobtainedreeding colony at the University ofromntheky).
To generate FeCγ25/APP−/− mice that would express AICD59 and Fe65 on APP null background, FeCγ25 hemizygous mice were bred with APP+/− mice to obtain FeCγ25/APP+/− mice in the F1 generation.
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