C57BL/6 and PD-1 deficient mice (obtained from Dr. Tasuku Honjo, Yoshida-konoe, Sakyo-ku, Kyoto University, Japan, via Dr. Megan Sikes, Transplantation Biology Research Center, Harvard Medical School, Boston, MA, USA) on a C57BL/6 background were bred in our specific pathogen-free animal facility at International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India.
ApoE−/− mice, also on a C57BL/6 background, were bred in house.
Mice on the C57Bl/6J background were bred in specific pathogen free conditions.
IFNAR1−/− mice on C57/Bl6 background were bred in house, female Balb/c, Bl/6 129 and C57/Bl6 controls were purchased from Harlan (Harlan-Sprague-Dawley, UK).
IFN-γ-deficient (IFN-γ-/) mice on the BALB/c background were bred in house from breeding pairs provided by Professor Casey T Weaver (University of Alabama at Birmingham, AL, USA).
GHRH-KO mice and their littermate controls (on a mixed C57BL6 and 129SV background) were bred in a closed colony at the RS's laboratory (Alba and Salvatori, 2004), housed under standard conditions (12-hr light/12-hr dark cycling and 20 23°C), and fed Lab Diet Formula 5001 (23% protein, 4.5% fat, 6% fiber) (Nestle Purina, St . Louis MO).
Tfr2−/− mice on the C57BL/6 background were bred in-house and have been described previously [23] All animals received humane care according to the criteria outlined in the "Guide for the Care and Use of Laboratory Animals" prepared by the National Institutes of Health [34].
Adult Oatp1b2 knockout mice and age-matched wild-type mice, both on a DBA/1LacJ background, were bred in-house.
Fluorescent yellow direct repeat (FYDR) mice and positive-control FYDR-Rec mice in the C57BL6 background were bred in-house.
Transgenic mice expressing Cre-EIIa on a C57BL/6 background were bred with homozygous Cryaa knock-in mice to delete the neor gene.
SR/CR mice in the C57BL/6 congenic background were bred at the ARP facility of WFU [ 1].
