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Age and sex matched wild type (WT) and PECAM-1−/− littermates on the C57BL6J background were bred from heterozygotes.
SASP+/− mice of a C57BL/6J background were bred from six generations to a pure Hos/HR-1 background through the speed congenic services of the Central Institute for Experimental Animals (Kawasaki, Japan).
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IFN-γ-deficient (IFN-γ-/) mice on the BALB/c background were bred in house from breeding pairs provided by Professor Casey T Weaver (University of Alabama at Birmingham, AL, USA).
IFNAR1−/− mice on C57/Bl6 background were bred in house, female Balb/c, Bl/6 129 and C57/Bl6 controls were purchased from Harlan (Harlan-Sprague-Dawley, UK).
ApoE−/− mice, also on a C57BL/6 background, were bred in house.
Mice on the C57Bl/6J background were bred in specific pathogen free conditions.
All mice were bred from the same background strain (129/Sv × C57BL/6J crossed with an outbred MF1 strain, more than 10 generations ago) (de Felipe et al., 1998).
Experimental animals were bred from parents, which were F2 after hemizygous mating and being on a C57Bl/6N background.
Ephrin-A2 -/- mice were bred from heterozygous parents at the Biomedical Research Facility (The University of Western Australia) and backcrossed for >10 generations on a C57Bl/6J background.
The Nat2 ∗null allele was bred from a129/Ola background onto two different genetic backgrounds (A/J and C57Bl/6, supplied by Harlan, UK) by backcrossing over ten generations.
This movement was bred from the human desire for immediacy.
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