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Exact(19)
Spot intensities were extracted and background was subtracted using GenePix Pro 6.0.
The background was subtracted using a control which contained only the cells and the dye.
DHE oxidation can occur due to basal macrophage metabolic activity [65], therefore background was subtracted using uninfected cells as control.
Images were cut, background was subtracted (using an area outside the cell), overlays were created and brightness/contrast was adjusted with ImageJ and PowerPoint software.
The background was subtracted using Tougaard's approximation.
Spot intensities were extracted and background was subtracted using TIGR Spotfinder 2.2.4 [ 44].
Similar(41)
Averages of the mean fluorescence of the entire embryo were calculated to compare expression levels and backgrounds were subtracted using identically sized areas from an empty region of the slide.
The collected spectra were averaged and the background fluorescence was subtracted using an asymmetric least squares smoothing.
Background signal was subtracted using the default rolling ball parameters.
The background image was subtracted using Adobe Photoshop (Adobe Systems, San Jose, CA) to eliminate the shadowing effect.
The mean intensities of small cellular areas of interest were collected as F t) and the background intensity was subtracted, using a same-size region of interest outside the cell, yielding F t)s.
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