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Background was substracted from raw data and infected cells were normalized to 100%.
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As previously described, regions of interest were drawn on the vascular wall to determine calcium signal from smooth muscle cells and background was substracted using MetaFluor (Universal Imaging Software) [17].
The background was substracted by using the negative isogenic MC4100 Hfq− strain.
Subsequently, local background was substracted and raw data were normalized and t-test performed for three replicates.
The intensity of the background surrounding each spot was substracted from that of each of the spots.
Background activity of sample control without D-galacturonic acid was substracted from sample measurements with D-galacturonic acid.
The initial scattered light intensity (I0) was mainly attributed to factors such as the media background or the type of the MTP and thus was substracted from the residual scattered light data (I-I0) [ 30] or fluorescence data (where appropiate).
The average OD of the reaction mixture in the absence of compost was substracted from the OD of mixtures containing fungal colonized compost or pure mycelium.
The remaining fluorescence in the bleached region after bleach was substracted from all data points.
This offset was substracted from the potential field in order to be able to compare the different configurations.
The mean RLU value of the negative control was substracted from the mean RLU value of each test sample.
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CEO of Professional Science Editing for Scientists @ prosciediting.com