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Background was set at 3 standard deviations above the average A405 readings of 3 wells to which no primary antibody was added.
The significance level for tests related to patient background was set at 15% for both sides.
Average tissue background was set at the minimum on a gray scale of 255 shades.
The experimental background was set at around 7.5 and an intensity of less than 9 corresponded to low expression.
The resulting expression levels for each of the miRNAs measured by the microarrays were calculated and filtered for miRNAs expressed above a background level (background was set at 30, approximating the median signal per array).
Arbitrarily, the intensity of the region prior to bleaching was set at 1 while that of the background was set at 0. Relative intensities are represented as a function of time.
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The numbers of clusters in the GMM for the SP, NSP, and background were set at 5, 10, and 2, respectively.
The background threshold was set at twice the background fluorescent signal plus 3 standard deviations.
During the examination, background illumination was set at 1.27 cd/m.
The background threshold was set at a level of 3-fold higher than the negative control.
The background threshold was set at a level three-fold higher than the negative control.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com