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To measure the observed intensity of the reporters in individual cells, regions of interest were drawn around the entire soma of a RGC expressing AKAR, and the average intensity of CFP, YFP and fura-2, less the background, was recorded for each time point.
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All clearly identifiable nuclear staining beyond background was recorded as positive for MCM-2 and MCM-5.
With the electrode at reference (bath) position approximately 1 cm from the cornea, the background value was recorded for a few seconds.
Background EEG was recorded for 5 min during sleep and/or while the patients lay motionless with their eyes closed.
The proteins were extensively dialyzed to the same buffer used for NMR, and the SAXS profile for the matching buffer was recorded for background subtraction.
For the morphological background, a T1-weighted MPRAGE was recorded for each control subject (TR 2500 ms, TE 4.32 ms, matrix size 256 × 256 pixels, 192 slices, slice thickness 1.0 mm, and in-plane pixel size 1.0 × 1.0 mm).
The lowest value ((K=0.679)) was recorded for the dimension Gap-Background classifier.
The mean pixel intensity was recorded for each scored animal after background subtraction.
Then, the Ne atom beam is delayed with respect to the NO packet, such that only background signal is recorded for 100 shots.
Background characteristics were recorded for both the women and their partners.
Current and past medical histories, personal backgrounds, and medications were recorded for all subjects.
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