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The introduction of erg26 -1 and ero1 -1 alleles into W303 strain background was performed using a strategy described previously (Li et al. 2011).
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Background subtraction was performed using the Bayesian background subtraction method, which permits negative background-subtracted intensities (BSIs).
Background correction was performed using the subtract background command with the rolling ball radius set to 50 pixels.
Background subtraction was performed using the 'Subtract Background' tool (rolling ball radius 30 40 pixels), and the 'StackReg' plugin was used to correct focal drift of the sample.
Background correction was performed using the Affymetrix Robust Multi-array Analysis (RMA) background correction model (Irizarry et al, 2003).
Background normalization was performed using Illumina's software to minimize the amount of variation in background signals.
Background selection was performed using 65 well-distributed SSR markers to preserve most of the KDML105 genetic background for the adaptability and market quality of the Thai Jasmine rice (Table 2).
For mouse arrays, background correction was performed using the 'normexp' method implemented in the Bioconductor LIMMA package to adjust local median background estimates (54).
Background subtraction was performed using Athena [44] and IFEFFIT [45] with a frequency cutoff parameter (Rbkg) set to 0.8.
Background subtraction was performed using a region of interest placed as close as possible to the sperm of interest.
Background subtraction was performed using the mean optical density value from 4 arbitrarily selected, vessel-free, areas (60×60 pixels).
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