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Upon completion, the gel was stained in Coomassie Blue staining solution until a clear background was obtained for scanning with GS-800 calibrated densitometer (Bio-Rad, USA).
The median of the pixel intensities for each spot and for the corresponding surrounding area (background) was obtained for each channel.
GFP background was obtained for each well from the promoterless control strains U427 (for strains U424 and U426) and U66 (for strain U428) (Additional File 1, Figure S1).
Library quality was similar to that of DsRed/mRFP – Figures 6(a) and 6(b) show that no parental background was obtained in the case of RD-PCR (23 sequences) and approximately 20% parental background was obtained for DNA shuffling (20 sequences).
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All promoter sequences in the database of transcription start sites [ 21] corresponding to genes with Pearson correlation above a "high" threshold (0.8, test) or below a "low" threshold (0, background) were obtained for analysis.
Although collagen can autofluoresce, good negative controls with very low background were obtained for the FITC immunofluorescence used to quantify either TG2 protein or the cross-link using cryostat sections.
Generation N6 Mstn+/+ and Mstn−/− mice [16] in the C57BL/6Ncr genetic background were obtained from homozygous matings for all experiments except diet experiments in which N6 Mstn+/+ and Mstn−/− mice were obtained from heterozygous matings.
For expression data, data corrected for local background were obtained from Illumina's proprietary software GenomeStudio.
To reduce variability in the phenotype analysis, a more defined genetic background was obtained by backcrossing Ugt1 mutant mice for more than nine generations to wild-type (WT) C57BL/6 mice, reaching at least 99.8% of C57BL/6 genetic background.
Polyvinyl butyral-carbon black composite electrodes coated on brass were fabricated to solve this problem and a fifty-fold lower background emission was obtained for these novel composite electrodes in comparison to that of oxide-covered 99.9% pure aluminum electrodes.
No background absorbance was obtained for the extracts and MTS in the absence of cells, as some extracts are capable of reducing the MTS.
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