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The background was obtained by measuring the fluorescence intensity of regions out of the cells.
The background was obtained by blocking the reflection of the RM.
A notable recovery of native genetic background was obtained by minimising coancestry with the exogenous individuals calculated from information on marker genotypes.
To reduce variability in the phenotype analysis, a more defined genetic background was obtained by backcrossing Ugt1 mutant mice for more than nine generations to wild-type (WT) C57BL/6 mice, reaching at least 99.8% of C57BL/6 genetic background.
Radioactive background was obtained by incubating the same amount of RBC or IRBC at 4°C and was deducted from the results obtained at 37°C so as to determine only the temperature-dependant uptake.
Radioactive background was obtained by incubating the same amount of free parasites at 4°C and was deducted from the results obtained at 37°C so as to measure only the temperature-dependant uptake.
Similar(54)
Cbs+/− mice, on a C57BL/6 background were obtained by mating male Cbs+/− mice with female wild-type C57BL/6 (Cbs+/+) mice.
PP1γ2 transgenic positive mice in a PP1γ −/− background were obtained by first crossing transgenic founder mice with PP1γ −/− CD1 female mice or PP1γ +/− male mice, then intercrossing PP1γ2 transgenic/PP1γ+/− progeny.
New LEA mutants with a high erucic acid content genetic background were obtained by screening a large B. napus EMS mutant population with TILLING [ 10].
Elimination of the residual T and B cells in the γc−/− background is obtained by crossing these mice to the recombinase activating gene 2 (Rag2) deficient mice [ 6, 32].
These double transgenic mice (C57BL/6 background) were obtained by crossing homozygotic PS1M146L transgenic mice with heterozygotic Thy1-APP751SL (Swedish: K670N, M671L and London: V717I FAD mutations) mice (Charles River, France).
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