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A background was observed in all the spectral frames and could be fitted to a blackbody temperature of ∼ 2900 K.
As demonstrated by CD20 immunolabelling of human tonsils (Fig. 1d) and with bone marrow preparations immunostained for CD20, CD61 and CD68 (Fig. 2), no unwanted background was observed in tissue samples processed with the omission of protein blocking prior to incubation with primary Ab.
A strong fluorescence background was observed in the sample treated between 170 and 350 °C, probably caused by residual fragments of the organic matrix.
No staining above background was observed in Purkinje cells in both diabetic and control rats.
Strong staining without unspecific background was observed in a subset of samples.
The lowest activity comparable to the background was observed in the case of constructs pDEH7 and pDE2.
Similar(47)
A marked decrease in background is observed in the cleared sample.
Elevated levels of alkanes, three times greater than background, were observed in samples recovered from five stations.
However, since the green and red channels are not exactly the same, a slight background is observed in the difference signal data sets.
A total of 4, 5 and 6 peaks representing outlier SNPs with higher FST relative to the background were observed in scaffolds 1, 3 and 21, respectively.
These observations are in line with the observations in this study and raise the question why a higher CTC background is observed in patients with benign disease as compared to healthy controls.
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