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A second background was generated by a 3rd order Markov model (RSAT package [ 10]).
Cngb3−/− mouse line (on C57BL/6N background) was generated by targeting deletion (Deltagen Inc., San Mateo, CA) as described previously (31).
The Δ laeA mutant strain in the ATCC11414 background was generated by homologous replacement of laeA in the ATCC11414 Δ kusA derivative (Chiang et al. 2011).
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In addition to the mixed-background strain, Col1a1 r/r mice on a C57BL/6J background and 129P1/ReJ background were generated by backcrossing for at least 9 generations.
SOD1G93A mice on a B6 background were generated by crossing B6SJL-TgN SOD1-G93A 1Gur males (Jackson Laboratory) to B6 females for 4 7 generations (N4 N7), and then SOD1G93A mice on an Als2-null background were generated in a similar manner.
Creb1loxP/loxP mice on a C57Bl/6 genetic background were generated by gene-targeting as described previously [12].
The dmpk−/+ heterozygous mice on 129SV background were generated by Reddy et al. [13].
WT, and PRLR KO mice on a pure 129 genetic background were generated by heterozygous crossbreeding [19].
Parp1−/− mutant mice under a B6D2F1 hybrid background were generated by sequential backcrosses of Parp1+/− mice to C57BL/6J and DBA2/J [11].
Other double mutants, including ste12ssn8, ste20ssn8, cpk1ssn8, and SSN8 overexpression in the cwc1 mutant background, were generated by crossing the strains with appropriate genotypes.
All the color mutants in the strain Af293 background were generated by random insertional mutagenesis via Agrobacterium mediated transformation for this study.
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