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The background was determined using a regression-based background mapping method.
For each band the background was determined using a field of the same surface area directly above and below the band.
Background was determined using a regression-based background mapping method.
The non-signal background was determined using the median intensity value plus one standard deviation for the blank positions and negative controls, and the maximum value for all arrays was set as the "no signal" value.
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Background is determined using a regression-based background mapping method [68].
Specific RT activity and background were determined using the Mn2+ reverse transcriptase supplied with the kit.
The signal intensity of each spot and its local background were determined using GenePix Pro software (Version 6, Axon Instruments).
Differential targeting between miRNA targets and background were determined using two-sided Kolmogorov Smirnov tests.
The signal intensity of each spot and its local background were determined using an Array-Pro Analyzer (Media Cybernetics, MD, USA).
Graphical genotypes of 41 CSSLs in the Koshihikari background and 39 CSSLs in the Takanari background were determined using 141 DNA markers distributed evenly across the 12 rice chromosomes.
After exposure, the plates were read with an FLA-3000 instrument (Fujifilm Corp .. To quantify the radioactivity in the autoradiograph, regions of interest (ROIs) were set as shown Fig. 2, and the photo-stimulated luminescence (PSL) value for each ROI ((PSL − Background)/mm2) was determined using Multi Gauge version 2.3 (Fujifilm Corp).
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