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The signal/background ratios for hybridizations using between 150 fmoles to 1.5 amoles of miRNA target was calculated and shown in Figure 2. Background was determined as an average of intensities from spots containing an unrelated control miRNA probes.
Maximal reduction of XTT was determined as the mean of three wells containing tumor cells only, and background was determined as the mean of three wells containing RPMI 1640 medium, 10% (v v−1) FCS.
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The relative quantity was determined as following.
The background was determined by interpolation between 14 data points.
The background was determined using a regression-based background mapping method.
Background was determined using a regression-based background mapping method.
The background was determined by quantifying the intergenic regions for each sample and taking the value of the 95th percentile as background expression (Additional file 2).
Due to the high and inhomogeneous background, the local background was determined from repeated dilation of each telomere segment to define the asymptotic value for the averaged intensity out of the segments as the background value.
Racial/ethnic background was determined by subject self-report.
Background was determined by using various isotype controls (BD).
Experimental background was determined by using empty wells with medium.
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