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A local background was calculated for each spot as the median values of the fluorescence intensities of 4 squares surrounding the spot.
Background was calculated for each array by the average signal of the negative control probes.
After each experiment the signal ratio (340 nm/380 nm corrected for the background was calculated for each pixel.
The number of PCR cycles (CT) required for the signal intensities to exceed a threshold just above background was calculated for the test and reference reactions.
A local background was calculated for each spot as the median values of the fluorescence intensities of four squares surrounding the spot.
The average TMRM fluorescence intensity of at least 15 ROIs minus background was calculated for each frame and normalized for comparative purposes.
Similar(53)
The distance, or the number of base changes that are necessary for a candidate probe sequence to be "converted" to the nearest sequence present in the synthetic background, is calculated for each probe.
A mean fluorescence background is calculated for the LY and PI fluorescence channel from the 3 control images in each petri dish and the detection threshold is set at the mean plus one standard deviation.
Background was calculated from average DxAm and DxDm values for rdr6-11 leaves (n = 20).
For this, a static background is calculated from a small number of initial frames.
For each array, the background was calculated as follows: log2 median intensity value for negative control probes + (3 * log2 intensity value standard deviation negative control probes).
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