Sentence examples for background was blocked using from inspiring English sources

Endogenous peroxidase activity was quenched by applying 3% hydrogen peroxide in methanol (BDH) for 10 min, and excess background was blocked using a solution of 5% normal horse serum and 1% normal goat serum (DAKO) for 20 min.

Non-specific background staining was blocked using 1 × casein in TBS for 45 min for pAktser473.

Non-specific background staining was blocked using 1.5% (v v−1 ) normal horse serum in tri-phosphate buffered saline and incubated for 20 min at room temperature.

Nonspecific background staining was blocked using either 2.5% horse serum in TBS for 20 min (PI3K and pAR), in 1% casein for 10 min (Akt1 3, mTOR and pmTOR) or in Serum Free Block (Dako A/S, Glostrup, Denmark) for 10 min (pAkt).

Non-specific staining was blocked using Background Sniper (Biocare Medical, Concord, CA, U.S.A).

After antigen retrieval, the slide was blocked using Background Sniper (#BS966M, Biocare, Concord, CA) for 20 min and stained overnight at 4°C with rabbit anti-human PIAS3 antibody (Cell Signaling Technology) at 1 400.

Endogenous peroxidase activity was blocked using 3% hydrogen peroxide and non-specific background staining was blocked using 5% horse serum in TBS for 20 min.

Endogenous peroxidase was blocked using 3% hydrogen peroxide for 20 min and non-specific background staining was reduced for SK1 by blocking with casein for 20 min and for S1P4 by blocking with 1 : 20 horse serum for 30 min.

Antigen retrieval was achieved by microwaving sections for 20 min in 10 m M citrate buffer pH 6.0, and endogenous biotin activity was blocked using a biotin blocking kit (Vector Laboratories Ltd ,Peterborough, UK) to prevent non-specific background staining.

Sections were blocked using Background sniper (Histolab, Göteborg, Sweden).

The slides were blocked using 10% casein solution for 1 h to remove background staining.