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The background was acquired using a gold mirror as reference sample.
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Arterial phase was acquired using bolus tracking.
Data was acquired using a logarithmic scale.
Data was acquired using SOFTMAX®Pro software.
Background (dark) images were acquired using opaque cardboard in one of the filter wheel positions.
Spectra were acquired using air background correction.
As the background magnetization approaches the null point, images can be acquired using a single-shot fast spin-echo sequence.
In each experiment, coverslips were viewed with a 63× objective Zeiss LSM510 fluorescence microscope and confocal images were acquired using identical photomultiplier settings and corrected for background fluorescence using unlabelled specimens.
Background fluorescence was set up by negative controls and data (10,000 cell fluorescence events) were acquired using a FACSCalibur™ flow cytometer (BD Bioscences, NJ, USA) and analyzed by CellQuest™ software (BD Bioscences).
The instrument was calibrated externally according to the manufacturer's instructions and all data were acquired using internal lock mass calibration on m/z 429.088735 and 445.120025 (background ions).
Images were acquired using an Agilent array scanner and spot signals were processed (default background subtraction) and quantified using GenePix Pro 4.1.
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