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Background values were removed and to ensure linearity, assays were used at below 50% saturation.
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Data were background corrected, flagged values were removed, spots in which both signals were <100 were filtered out, ratios were log base 2 transformed and lowess intensity-dependent normalization was used to adjust for differences in labeling intensities of the Cy3 and Cy5 dyes [ 46].
After background correction, negative expression values were removed and the data were normalized by scaling all individual intensities to a fixed target.
Those values were removed from Table 2.
These outlier values were removed before calculation.
Missing values were removed.
Prior to the analysis, probe-sets with detection above background (DABG) p-value were removed from the input data.
Each duplicated value is removed (Lines 5-8).
Assays were done in triplicate, and background values were subtracted.
Background values were not subtracted.
Background values were subtracted from the measured intensity values.
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