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The signal intensities of the spots and background values were measured using array vision 7.0 (array vision, USA).
Local background values were measured in the corners of spots and were subtracted from the signal intensity values for each spot.
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A mean background value was measured (B1) and subtracted from the expression value.
Absorbance values were measured with SpectraMax Plus384Spectrophotometer.
Initial OD600 values were measured and recorded.
Anthropometric values were measured.
If cluster densities are reduced, the background signal from clusters in close proximity is reduced which leads to higher intensity over noise values being measured.
British values are measured in pounds and pence.
For every probe, RFU-values were measured every cycle and after background normalization plotted against the cycle number.
Results are expressed as % bound fB or properdin using the following formula:
Background values were subtracted from the measured intensity values.
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