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Median density values and background values of each spot were extracted for both the experimental samples (Cy5) and the reference samples (Cy3).
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Cellular oxidative capacity of the LAC, PYR, or BHB was calculated by subtracting the background value of each substrate and standardized by protein contents.
The lower detection limit of the assay was defined by adding 0.1 OD units (rounded 8-fold mean of the standard deviation of background values from eight plates) to the background value of each plate.
Software settings were kept the same for every image analyzed within one experiment, background values of 2 to 6 were subtracted from each image before processing.
Final concentrations were acquired by subtracting the background values of the flux from the calculated values by the Glitter software.
This selection may depend both on their fit with the facts and on their fit with the background values of the storyteller.
Contents of all heavy metals present in the study area were considerably higher than their background values of provincial and national standards except for As.
The background values of the pollutants were below the maximum limits for drinking and irrigation water and with no need of reactors, collectors, aeration or recirculation systems.
Aside of these local anomalies, heat flow variations are restricted to near background values of 50 70 mW/m2, except in the uplifted Academician zone.
The background values of free mercury 0Hg) seemed to be more stable and the anomalous zones narrower than those of radon gas in the fault zone [24].
Compared with the background values of heavy metals in virgin forest soil, concentrations of Mn2+; Cr2+; Pb2+; Fe3+; and Cd2+ were substantially enriched in the study sites.
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CEO of Professional Science Editing for Scientists @ prosciediting.com