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In the case of the construct, "background values" from the wild type domain were subtracted from levels in the expression domain to remove noise from the dataset.
The background adjustment was performed by subtracting median background values from the median expression values obtained by FE and then converting the results to log-scale.
The results were calculated by subtracting the mean background values from the values obtained under each test condition and were expressed as the percentages of the control (untreated cells) values.
Similar(57)
Caspase-3 activity levels were calculated by subtracting the background value from the sample value.
The final mean fluorescence intensity was calculated by subtracting the mean background value from the mean signal value.
Expression values for each feature and dye channel were obtained by subtracting the median of the local background value from the median of the foreground value.
After determining the fluorescence intensities of both channels, a background correction was made by subtracting the local background value from the foreground intensity.
The mean gray intensity of the entire image was also calculated in ImageJ and used to subtract an average background value from the invaded spot.
The results were calculated by subtracting the mean background value from the values obtained under each test condition: values were normalised to the number of cells in each well and were expressed as the percentages of the control (untreated cells) values.
The lower detection limit of the assay was defined by adding 0.1 OD units (rounded 8-fold mean of the standard deviation of background values from eight plates) to the background value of each plate.
Percent inhibition of CETP activity was calculated by subtracting the background values from both control and test sample values.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com