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In each image, the background value was set using the "threshold" function of the software to exclude all pixels surrounding the positive stained signals.
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The average A and the standard deviation SD of background signal was calculated and the threshold value was set at A + 3SD.
Where background was higher than the ChIP-seq signal, the value was set to zero.
The minimum fold change value was set to 2. Resulting genes were classified according to their functions against the background of gene-ontology databases and the underlying literature and experimental background.
The p value was set at p<0.05.
The background threshold value is set to be: average background counts + standard deviation of background counts * sigma_factor.
A background value was used to set the threshold between labeled pixels and background pixels.
Flag value is set to 1 if the spot has an intensity value significantly different from the local background, 0 otherwise.
Spots with a mean intensity below their local background were excluded and the normalized M value for missing values was set to zero.
The e-value was set to 1e-30.
(Missing values were set to 0).
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