Sentence examples for background to generate the from inspiring English sources

ApoE deficient mice (C57BL/6 background) were purchased from Jackson Laboratories (Bar Harbor, ME), mated with PDZK1 deficient mice (129 SvEv background), and backcrossed for 6 generations into C57BL/6 background to generate the PDZK1/apoE double knockout (dKO) and apoE single KO mice as previously described [21].

The following stocks were used in this study: Wn, an isogenised wild type strain, which was the strain used as a background to generate the rel20E mutant [7], rel20E [7], dif [6] and dif-key [14].

Abca1+/− mice were crossed to APP23 mice (C57BL/6 background) to generate the APP23/ Abca1+/− (APP23/het) progeny.

Sequential mutagenesis steps were used against the ATC232S background to generate the novel double-cysteine residue mutants E162C/V170C and K191C/T339C, as well as K168C/F189C, S283C/P361C and S292C/T339C that have been reported previously [ 21, 30, 31].

First, we deleted one allele of SEC6 in the THE1 background to generate the strain sec6Δ/+ via a PCR-based gene disruption strategy previously described by Wilson et al. (21), using primers SEC6-5DR and SEC6-3DR and plasmid pDDB57 as a template.

In brief, Nestin-cre or Emx1 cre mice were first crossed to the MADM11-GT line (on a 129X1/SvJ background) and the resulting MADM11-GT Nestin-cre or MADM11-GT Nestin-creMADM11-GT Nestin-creed torthe MADM11-GT + micEmx1lso on a 129X1/SvJ bacreround) to generate the mice11-TG/GT:Nestin-cre or MADM11-TG/GT:Emx1 cre mice for mosaic analyses.

The progeny were crossed into the rd10 background to generate mice homozygous for the rd10 mutation and heterozygous for CX3CR1CreER and DTA (rd10/CreDTA mice).

We crossed these mice into the rd10 background to generate rd10/CreDTA mice and depleted the retina of microglia by tamoxifen administration at P21 23.

Several efforts have been made to use E. coli K4, or its biosynthetic machinery in a different background, to generate cell factories for the production of chondroitin, and encouraging results were obtained (Cimini et al. 2013, 2015; He et al. 2015; Jin et al. 2016).

To test this, we generated further Ngn3 mutants in which cysteines were mutated to alanines either in a wild-type background to generate Ngn3CO or in the presence of additional mutations of canonical ubiquitylation sites to generate Ngn3KOCO and AcNgn3KOCO.

Mutant lines were backcrossed for at least ten generations to the C57/BL6 background to generate breeding stock for these experiments.