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First, a binary value was declared for each transcription factor (TF) in each sample as expressed/unexpressed, based on the results of the cDNA macroarray (background threshold was considered as 0.01 for this analysis).
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The multimodal histogram contained one large peak around 1 (defined as the background response) and neurons with fold-changes greater than the first minimum (defined as threshold) were considered responsive.
Speed threshold is considered Vth = 5 m/s.
Because of a high background reactivity observed for this cohort, we additionally stipulated that for positive samples, the optical density ratio (ODR) of viral protein 2 to control must be >1.2; ODRs below this threshold were considered negative.
A fluorescence background from untreated cell is determined for each petri and only treated cells that shows fluorescence above one standard deviation (calculated from the 3 control images) from this threshold are considered successfully perforated.
Building regulation and daylight thresholds are considered as design constraints.
A background threshold was then estimated.
The background threshold was set at twice the background fluorescent signal plus 3 standard deviations.
Values above a background threshold are recorded as positive.
With that background, Mr. Husseini was considered Palestinian nobility.
Methylation below the threshold level of 10% was considered background (Nygren et al, 2005).
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