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Background subtraction was done for each lane at positions where no visible spots could be seen.
To subtract large background artifacts, an average of all images was subtracted from each image, and further background subtraction was performed by rolling ball subtraction in ImageJ.
For all images, beside those of actin, local background subtraction was done by high-pass filtration as previously described [11, see Fig. 1 there].
Background subtraction was performed using the Bayesian background subtraction method, which permits negative background-subtracted intensities (BSIs).
In all the measurements, background subtraction was made.
No background subtraction was performed.
After deconvolution, a background subtraction was performed followed by the colocalization analysis compiled from both channels.
A response greater than 0.05% after background subtraction was considered positive.
Background subtraction was utilized.
Background subtraction was selected.
Background subtraction was performed online.
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