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The microarray data were normalized without performing background substraction, using the global Lowess method performed with the Goulphar software program [ 41].
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Following background substraction, the resulting expression measurements were corrected for spatial and intensity biases using algorithms from the MatArray toolbox (Venet, 2003).
The data were normalized without background substraction by the global Lowess method performed with the Goulphar software [34].
Background substraction were carried out using normexp.
Currently, most successful approaches to human detection are based on background substraction techniques that apply only to the case of static cameras or cameras with highly constrained motions.
SSH was performed using the PCR-select cDNA substraction kit (Clontech) according to the suppliers instructions.
The raw intensity data was obtained from the scanned arrays using the BeadStudio software version 1.4.02 (Illumina, San Diego, CA, USA), using the default probe summarization and background substraction methods.
The gel images were analyzed using SameSpots (Nonlinear Dynamics) involving automatic normalization and automatic background substraction.
Image processing (background substraction, cell segmentation) could be integrated within the same master program.
The autofluorescence background substraction procedure was standardised on tissue slices from five uninjected animals.
* Background: the authors use "background", "background lights", or "stimulus" to describe changes in the background illumination.
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