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The background staining was evaluated using a control isotype labeled with phycoerythrin (BD Bioscience Pharmingen) (data not shown).
Background staining was evaluated in cells incubated with an isotype control immunoglobulin G (IgG) followed by anti-rabbit IgG Alexa 488-conjugated antibody.
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Only cytoplasmic staining was evaluated.
Immunohistochemical staining was evaluated by a pathologist.
Immunohistochemical staining was evaluated by visually assessing staining intensity (0 3).
Intensity of the staining was evaluated separately.
Immunopositive staining was evaluated in five areas.
Only unambiguous membrane staining was evaluated.
Cytoplasmic staining and nuclear staining were evaluated separately.
Membranous and cytoplasmic staining were evaluated.
Both cytoplasmatic and nuclear staining were evaluated.
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