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The background staining was eliminated by a mouse-on-mouse kit (Vector, Burlingame, CA).
Background staining was eliminated by testing antibody dilutions (1 : 100, 200, 500, 1200 till 5000) while tumour-specific signals of TRIM59 proteins were noticeable.
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Background staining was done with propidium iodide (Invitrogen).
Background staining was done with propidium iodide (Invitrogen, Taastrup, Denmark).
No background staining was detectable in mammary tissue when lacZ staining was performed.
Very little background staining was detected.
Background staining was assessed using isotype controls.
At this concentration, no non-specific background staining was detectable.
Non-specific background staining was reduced by protein blocking agent.
In all cases, background staining was extremely low or nonexistent.
No interfering background staining was observed (data not shown).
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