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Hybridization was done following protocols previously reported.[53] Details of all probe synthesis, sequences, and specificity are described elsewhere.[53] Background staining was done to all FISH slides with a DNA stain, DAPI (4',6-Diamidino-2-Phenylindole Dihydrochloride).
Background staining was done with propidium iodide (Invitrogen).
Background staining was done with propidium iodide (Invitrogen, Taastrup, Denmark).
The background staining was done with Mayer's haematoxylin.
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DAPI staining was done to stain the nucleus.
Nuclear staining was done by H33258 Sigma-Aldrich, Saint Louiss, MO, USA).
Immunohistochemical staining was done as described [30].
Nuclear staining was done with methylene green.
DAPI staining was done following standard techniques.
Masson's trichrome staining was done to stain collagen fibers.
To stain cells for actin, phallotoxin staining was done.
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