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To cross-compare staining signals between cell lines, the mean fluorescence intensity (MFI) of the background staining was adjusted to around 10, and the relative staining signal was expressed as the ratio of sample MFI divided by the control MFI.
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An increase in the background staining was observed on the recto of the insufficiently washed samples.
Background staining was done with propidium iodide (Invitrogen).
Background staining was done with propidium iodide (Invitrogen, Taastrup, Denmark).
No background staining was detectable in mammary tissue when lacZ staining was performed.
Background staining was assessed with an isotype-matched control monoclonal antibody.
Very little background staining was detected.
Background staining was assessed using isotype controls.
At this concentration, no non-specific background staining was detectable.
Non-specific background staining was reduced by protein blocking agent.
In all cases, background staining was extremely low or nonexistent.
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