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In addition, minimal background staining was achieved when cyclin D1 primary antibodies were detected using the EnVision system (DAKO, Ely, UK).

Nuclear staining was achieved with haematoxylin.

Successful TIMP-1 staining was achieved for 291/321 cases.

Nuclear staining was achieved by adding DAPI/Rnase-A.

Visualization of the staining was achieved with DAB.

Nucleoid staining was achieved with 20 μg/mL DAPI.

Nuclear staining was achieved with 4′,6-diamidino-2-phenylindole (DAPI).

Staining was achieved with DakoCytomation EnVision System-HRP (DAB) kit.

Coomassie staining was achieved using GelCode® Blue Stain Reagent (from Thermo Scientific).

Nuclear transcription factor staining was achieved using the forkhead box protein 3 (FoxP3) Staining Set (eBioscience).

Counter-staining was achieved with hematoxylin (blue).