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IgG control stained vessels revealed no background staining (Figure S4).
Samples treated with CCCP represent background staining.
Faint signal in the pharynx and central body region in egf-6 is background staining.
The same procedure was applied to the background staining control surfaces.
The current protocols for blocking background staining in immunohistochemistry are based on conflicting reports.
Here you can clearly see the GABAergic neurons with almost no background staining.
Kim, S. H. et al. An improved protocol of biotinylated tyramine-based immunohistochemistry minimizing nonspecific background staining.
Black curves represent the background staining obtained after treatment of samples with the mitochondrial membrane potential disrupter CCCP.
In recent years, we have noted higher levels of background staining in the IFA test for PHF (Neorickettsia risticii).
Values of <200, <400, and even <800 are due to the background staining at the lower dilutions.
As a negative control, each sample was also stained with antibody pre-adsorbed with peptide to assess background staining.
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