Exact(11)
Immunogold background stain was only rarely observed in non-infected HFH and Huh-7.5 gel tissue (Fig. 9E and 9F).
The threshold intensity was set to include those cells that were seen by eye to be positively stained for SNCA and to discount the surrounding background stain.
Furthermore, during analysis of borderline-concentrated HCMV-reactive sera in classical neutralization tests, like conventional micro-neutralization assays, high concentrations of IgG and serum proteins can cause an unspecific background stain of the cell monolayer (Reinhard H., unpublished observation).
Lower panel shows staining of uninfected LapC4 cells with anti-gC antibody in order to prove its specificity for infected cells (Texas red is the background stain of the anti-gC antibody preparation and serves to visualize the cell monolayer).
The background stain was subtracted in each histology analysis.
As controls, sections without the primary antibody were used to evaluate the non-specific background stain.
Similar(49)
IgG control stained vessels revealed no background staining (Figure S4).
The TUNEL-positive nuclei are clearly visible (dark stained nuclei) over the background staining.
Samples treated with CCCP represent background staining.
For background staining, 0.05 % neutral red was used.
The current protocols for blocking background staining in immunohistochemistry are based on conflicting reports.
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