Sentence examples for background signal using a from inspiring English sources

Sequencing data were first filtered to identify reads significantly (p<0.05) enriched over background signal using a Poisson-based test.

Absorbance was measured at 450 nm (subtracting 570 nm background signal) using an UVM340 plate reader with DigiRead software (Asys Hitech, UK).

Once the optimal probe concentration and hybridization conditions were optimized to reduce the background signal using untargeted probes (Figure S6 A and B), we performed FISH and stained with TIAR or DCP1, after rinsing abundantly with phosphate buffered saline to remove excess formamide.

Negative control sections demonstrated minimal background signal using either antibody (Fig. 5 Ca d ).

However, unlike direct atmospheric measurements, these background scans spanned a small time window relative to the length of the enrichment experiment, making it difficult to characterize evolution of the background signal using the background scans alone.

After the adjustment of background signal using the Plier method, probe intensities were normalized using the quantile normalization procedure with Affymetrix Expression Console software [ 66].

In mouse NIH3T3 cells, we failed to obtain significant enrichment for the chromatin-bound PCNA relative to the background signal using the same antibody (data not shown).

The ratio between GFP/Venus and mCherry emission intensities was quantified for every cell expressing mCherry after subtraction of the background signal using the Sca ;R analysis software as previously described.

Raw data were corrected for background signal using the RMA (Robust Multi-array analysis) algorithm (affy_1.22.0 package of BioConductor) that normalizes the data so that different arrays can be compared to each other and summarizes the data into expression values [ 28].

The Li1s core level spectra has been corrected for any background signals using the Shirley algorithm prior to curve resolution and then resolved into two components centered at ~54.8 and 56 eV as shown in inset of Figure S5.

Data were analyzed by first subtracting the background and then normalizing the signal using a locally weighted regression filter.