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We also performed enrichment tests as described in the Methods section, to ensure that conserved residues are enriched in functional residues when compared to the background set of residues.
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The hypergeometric distribution was used to assess enrichment between the background set of probe-sets and a sample set.
We also needed to construct a background set of non-pre-miRNA hairpins to estimate the background noise.
The set of residues which can be mutated to broaden specificity might therefore not coincide with the set of residues which detect differences in DNA sequence.
Helix interface is defined as a set of residues that are in contact with residues from the other helix.
Let Epi denote the set of residues in a putative epitope and Ab the set of residues supplied as the binding site on the antibody.
Contact graph: G*=(V*, E*), where V* is a set of residue positions and E* is a set of pairs of nearby residue positions.
Our background set consists of 1000 sequences also from dbTSS.
We have performed enrichment tests, for both FunFam and FineFam functional families, to ensure that conserved residues are enriched in functional residues compared to the background dataset of residues, i.e. all residues in the proteins.
In the D38E background, mutation of residues 42 and 44 led to 2 3-fold rate reductions, slightly smaller than in the WT background, suggesting only a small role of these residues in general base positioning.
We started by analysing how the choice of background set for overrepresentation analysis would influence the clustering result, using three different background sets.
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