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The middle phase, containing the concentrated proteins (see "Background" section), was used for further study.
This property – most probably associated with its role in placentation, see the Background section – was used to characterize further the consequences of the identified SNPs on the fusogenic function of the encoded proteins.
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The other two sections are used as background reference spectra.
7 μm frozen sections were used.
Pooled sections were used for RT-PCR.
Local background subtraction was used to facilitate background correction.
No activity in the background was used.
We obtained the highest LRRK2 signal over background in immunohistochemistry experiments when freshly sectioned brain material was used the same day as perfusions.
Only the nuclear staining was considered positive, and only sections without background staining were used for analysis.
The same area of interest was used for all sections, and all values were corrected by subtracting the background value achieved from the cortex of each section.
Each entire unmanipulated field of view was blinded and quantified for the stain, whilst an unstained area was used to determine (and subtract) background staining for each section.
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