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[ 19] A point source was located centrally at 0.1 mm depth in a 15mm × 15mm domain (represented by a 100×100 pixel mesh), and the background scattering was set by defining the optical diffusion constant to be D = 1.5 mm.
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The prior means for absorption and scattering were set as μ̄ a = 0.01 mm-1 and μ̄′ s = 1mm-1, and the marginal variances for the inhomogeneity and background part were set such as 2 s.t.d.
Both forward scatter and side scatter were set at logarithmic gain.
Forward and side scatters were set to exclude dead cells.
The background scattering was subtracted from the sample scattering to obtain the scattering intensity from the solute molecules.
Background scattering was subtracted and data reduced, normalized and extrapolated to infinite dilution using PRIMUS (Konarev et al., 2003 ).
The background density was set to 0 g/cm3.
To detect small particles, forward scatter (FSC) was set to 600 and side scatter (SSC) to 550.
To avoid debris, electronic background, and undesired clumps in the bacterial samples, a Gate R1 was set up, which was based on light scatter for the flow cytometry assay.
Finally, the vessel absorption coefficient was set to μa3, and the same scattering coefficient as the background and inclusion.
limits for the contrast of the inhomogeneities corresponded to 40%% of the mean values for absorption and scattering and the marginal variances for the background part were set such as 2 s.t.d.
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