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Due to its semitransparency and bifacial activity, this cell type is very sensitive to background reflections and diffuse irradiation.
The subsequent coating with a thin boehmite layer decreases the intensity of the "background" reflections and enhances the optical visibility of the color print (Fig. 1B).
An absorber was placed behind the sample to prevent background reflections for optically thin samples.
Another concern regarding in vivo imaging is that background reflections from tissues lower the contrast of cells, thereby preventing the accurate counting of the cell number.
Background reflections from the cuvette walls have appeared to be an error source in other experiments, also, but smaller cuvette dimensions have smaller light paths inside the cuvette, and the signal may be less affected by the immersion medium [ 28, 29].
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The introduction of the anti-reflective layer decreases the "background" reflection and therefore improves the effective visibility of the target beam c.
Because of the high refractive index of the applied material and the relatively high reflectance from the interface between its surface and air (Fig. 1), we not only see a color image created by the interference in the thin layer, but also the background reflection from the air-surface interface (Fig. 1, Background and interface reflection).
There is a decrease in water background reflection from 550-750 nm; so to get some information on background the difference of bands 3 and 2 can be applied.
Both image classification techniques provided indistinct boundaries of LSMA sites and spectral confusion - common in areas of sparse vegetation cover due to high background reflection from the soil, as is the case in Butana - led to mislabeling of land classes.
The net reflection peak in the blue region (around 480 nm) is improved significantly from ∼40% to over 90% (by subtracting the background reflection of ∼7%), confirming the improved interference patterning introduced by the Ag mirror.
The colors in Figure 3(c) correspond to the spectrum with a peak position at 750 nm and while the main part of the reflection peak is in the infrared, the color is not fully homogeneous across the image and the broadband background reflection can also be observed.
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