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Absorbance was read at 490 nm, background readings were subtracted from the sample wells, and data were expressed as percentage of controls (sham-incubated cells).
Absorbance was read at 490 nm, background readings were subtracted from the sample wells and data were expressed as percentage of controls (A549 cells without neutrophils in the absence or presence of LPS).
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The background absorbance readings were subtracted from the readings assayed with AtAPY1-GFP.
Background (mock) readings were subtracted from the signal and values are expressed as percentage of untreated cells.
Absorbance readings were subtracted from a background measurement at 440 nm, which is the absorbance of culture medium with WST-1 in the absence of cells.
For the calculations, the average value of background group readings was subtracted from all of the sample readings.
The background reading was subtracted from the readings of the samples, and PARP activity was calculated using the standard curve obtained from the readings of standards.
Background readings were collected and subtracted from each spectrum before data output as described in Gonzalez et al. [14].
The absorbance reading was subtracted from the background control.
Background readings were taken from appropriate neighboring regions of the blot and subtracted from intensity measurements.
Background measurements were subtracted from readings.
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