Sentence examples for background readings from the from inspiring English sources

The background readings from the SDS solution were subtracted from each reading and the percentage of cells remaining after curcumin treatment was calculated.

"It's good to have the background readings from the computer," he said.

In this case, direct subtraction of the background readings from the uncorrected fluorescence intensity at each time-point t is not appropriate, as the size of the bacterial population generating the uncorrected signal is generally different from the size of the population generating the background signal.

Whole cell lysates were combined with Caspase-3 substrate reaction buffer and incubated for 3 h at 37°C and the absorbance was measured at 405 nm with a plate reader according to the manufacturer (Assay Designs, Ann Arbor, MI). Background readings from cell buffers and substrates were subtracted from the sample readings according to the protocol.

Similar dilutions were also prepared of the clarified lysate of E. coli cells carrying a null pCWori plasmid in order to assess the background readings from lysate without any P450.

Whole cell lysates were combined with Caspase-3 substrate reaction buffer and incubated for 3 h at 37°C and the absorbance was measured at 450 nm with a plate reader according to the manufacturer (Assay Designs, Ann Arbor, MI). Background readings from cell buffers and substrates were subtracted from the sample readings.

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After assay validation as described above, cut-off values were set at 2.1, which was two times the average absorbance readings of duplicate negative control sera after subtraction of background readings from a blank control well.

To calculate bacterial killing ability we first subtracted background absorbance readings from the absorbance readings (i.e., 12 and 24 hour readings).

Absorbance was read at 490 nm, background readings were subtracted from the sample wells, and data were expressed as percentage of controls (sham-incubated cells).

Absorbance was read at 490 nm, background readings were subtracted from the sample wells and data were expressed as percentage of controls (A549 cells without neutrophils in the absence or presence of LPS).