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The RTCA SP Station was connected to the RTCA control unit and the xCELLigence system was tested by resistor plate verification prior to the xCELLigence system being placed inside the incubator at a humidified atmosphere at 37°C and 5% CO2. Background quantification was done by adding 100 μl of the appropriate growth medium to the wells.
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All extracts were kept frozen until quantification was done.
Quantification was done against a calibration in the test medium.
Quantification was done by using benzoic acid as internal standard.
Quantification was done using NIH image.
Protein quantification was done by Bradford assay.
Quantification was done blindly by multiple researchers.
Quantification was done as detailed before [21].
Quantification was done using the ΔΔCT relative quantification method.
Quantification was done with the instrument software and the number of copies of DNA selected with target beads and (His 6 beads were compared to determine signal/background ratios.
Protein quantification was done as described [25].
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