Sentence examples for background of the yeast from inspiring English sources

To investigate the functionality of the human ortholog of the FMP43 protein in S. cerevisiae, we inserted the human gene in the genetic background of the yeast strain Δfmp43 (HUM).

To extend our findings to human cells and identify proteins that could serve as drug targets, we replaced the yeast FMP43 protein with its human ortholog BRP44 in the genetic background of the yeast strain Δfmp43.

Consistent with the notion that siderophore uptake is strongly dependent on the genetic background of the yeast strain [ 68], the present study also uncovered important variations in the arsenal of siderophore transporters encoded in genomes of different S. cerevisiae strains.

However, these studies were not performed using the BY4741 strain, which was the one used to screen the determinants of tolerance to furfural, vanillin and high glucose concentrations [ 16, 18, 19], and therefore they were not considered because the genetic background of the yeast strain used is known to have a high impact on the results obtained in large-scale phenotype screenings.

The genetic background of the yeast strains used in the present study is based on BY4741 Yeast whole cell extract (WCE) was prepared by glass beads vortexing in buffer A (25 mM Tris, pH 7.4, 5 mM MgCl2, 1 mM ATP 150 mM NaCl and 1 mM DTT) and cleared by centrifugation at 14000  g for 15 min.

For all Selders's concern, the tea and spices in it hovered politely in the background, leaving the yeast to run the show.

Conservation of bulk nucleosomes in the two complete genome datasets [ 7, 8] is comparable with background conservation in the yeast genome (0.71 for genic nucleosomes, compared with 0.73 for yeast genic regions).

It is based on the LC-MS analysis of samples composed of a fixed background of yeast lysate and different spiked amounts of the UPS1 mixture of 48 recombinant proteins.

The relative abundance of Ty elements in the genome is related to the genetic background of the strains [ 37, 56, 57] and yeast strains differ in their relative expression.

Both human orthologs were under control of the yeast promoter, and the original yeast genetic background was preserved.

The targeted phenotype is sometimes limited by its selectability (cf. infra), while the complex or understudied genetic background of certain yeasts can sometimes hamper their use in improvement strategies.