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We introduced a SopEM45-TEM-1 fusion into the background of the wild type, WTM−F−, and WTM−F+, respectively, yielding the strains WTTEM, WTTEMM−F− (ΔfliGHI), WTTEMM−F+ (ΔflgK).
We used the pPIN1 PIN1-GFPpPIN1 PIN1-GFP6] (in the baconstructof the wild type or fil-9 plants) as a marker for the meristematic L1 cell layer.
In Figure 7C the mutation was observed in the background of the wild type TP53 sequence; this likely indicates presence of an adjacent white blood cell in the WGA reaction.
The fact that the identity and the genetic background of the wild two-humped camel remain obscure means that designing and implementing conservation programmes for the wild camel are difficult.
For overexpession of PaGlo2 in the genetic background of the wild type, the gene including the terminator region was amplified using primers BamHI-Glx2-f (5'-AAGGATCC ATGCATATCCGGTCTATT CC-3') and HindIII-Glx2-r (5'-AAAAGCTT AGCGAA CCAGCGAATCCG-3').
To test if the observed signal originated from the cell wall, we used the same protocol to stain cell wall mutant cells (cw15 mt+, cw15 mt-, cw92 mt+, cw1 mt-, cw14 mt+ and cwd mt-) in the background of the wild type strains used for the initial staining.
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Interestingly, we detected a significant effect of background on the wild-type allele MenEx3 + under the experimental temperatures, but not at 25°.
Subsequent studies failed to validate this finding, however, probably because most of these studies focused on primary breast tumors instead of metastatic lesions and employed techniques that had low sensitivity for detecting rare mutations in the background of the wild-type alleles.
To increase the number of males we used a him-5 e1490) him-5 e1490 the background of the wild-type N2 strain in the following strains: unc-29(e1072), unc-38(sy576), tph-1(mutationlin-39(n1760), unc-64(e246), and unc-30(e191).
On the other hand, the low diversity in the wild boar may reflect a narrow genetic background of the Finnish wild boar population.
Moreover, the genetic background of the hybridizing wild and crop populations themselves can influence the phenotypes of hybrid generations and possibly selection dynamics (Mercer et al. 2006b).
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