Unfortunately such supposition is not supported by the available information on the genetic background of the hybrid varieties from KWS Lochow: Gonello F1 ((Lo115-P × Lo133-N) × LSR83 (Lo310 × Lo312)) is closely related to Guttino F1 ((Lo115-P × Lo133-N) × LSR81 (Lo298 × Lo312)), and the male line Lo310 is included in the pedigree of Palazzo F1.
Wu and T. Matsumoto, unpublished), indica variety 93 11 was considered to have a genomic background of the japonica variety around the Hd6 region, indicating the presence of gene flow between the two subspecies due to recent introgressions [36].
However, sorghum bmr hybrid varieties have been created that experience yields similar to wild type [ 113], suggesting that the genetic background of the mutant variety is important in overcoming yield reduction.
Data from these trials demonstrated the consistent performance of these lines, validating the effectiveness of SUB1 in conferring tolerance of submergence, independent of the genetic background of the recipient variety or the environment where it is grown.
To validate the association between the Pi2/9 haplotypes and resistance against rice blast, we generated BC3F3 introgression lines of Pi2/9-A15 Pi2/9-A152 and Pi2/9-A42 Pi2/9-A42ckground of the susceptible vandety CO39 via MABC using the marker of Pi2/9-A53 Pi2/9-A53the derined monogenic lines as IR126181, IR126183 and IR126184, respectheely.
The level of coverage of the wild introgressed segments in the background of the cultivated variety was optimal both in the BC1F1 and BC2F1 populations.
Marker assisted selection of BC1F1 and then BC2F1 lines carrying the desirable donor segment with the best possible return to the background of the cultivated variety provided a set of lines offering an optimal distribution of the wild introgressions.
This percentage is slightly inferior to the expected 25%, which is consistent with the selection that occurred at each generation for the best possible return to the background of the cultivated variety.
The rapid decrease of wild segment lengths observed between the BC1F1 and BC2F1 generations as well as the good level of recovery of the genetic background of the cultivated variety in BC2F1 generation is of great interest for the genetic mapping of QTLs and the development of Introgression Line ILlibrariesies.
To select a subset of BC1F1 and BC2F1 lines providing an optimal coverage of donor genome into the recurrent background, we imposed a target length of the introgressed wild segments of 20 cM, an overlapping of adjacent segments for a given LG and the best possible return to the background of the cultivated variety.
