The background of this experiment is a theory called the "global workspace" model of consciousness, according to which perceptual awareness involves two stages of neural activity.
After every third selection round an additional negative selection was performed to remove aptamer candidates binding to background materials of the experiment or to fecal specimen, the later sample matrix.
The background of the perfusion experiment was logged by selecting an identical region of the image plane, where no part of the sample overlapped.
The C5a-induced IL-8 concentrations were estimated by subtracting the background levels of IL-8 present in cultures not activated with C5a and pre-exposed or not to TLR ligands from the corresponding C5a-activated samples (background levels of the experiments described in Fig. 1B – typical of all experiments – are shown in the figure legend).
The field 'broodstock source' described the recent history of the population used in the experiment, or a measure of the genetic background of the population under experiment; 'treatment' described how the population was treated during the experiment, or the environment in which the population was tested.
To investigate the effect of background noise, the experiment was repeated for input SNR of 3, 8, 13, and 18 dB using the measurement data.
The background and description of the experiment, located under the Story tab on the website, are available in seven languages.
A systematically higher background in the experiment of Figure 3 figure supplement 2A would not increase the rate of initial uptake, which was measured over 4 time points.
A factorial design was chosen instead of a repeated measures design because there were few differences in experience, training, or background of the subjects in this experiment which makes it unlikely that real differences between treatments would be confounded with inter-subject variance other than their perception of the CRE, which could not be separated by a repeated measure design.
The background, description, and status of the experiment are given.
