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nucleatum more than 3 standard deviations above the background of the assay.
Serum antibody titres were designated as the reciprocal of the dilution of antibody that gave an OD492 value five times the background of the assay.
No CD45.2+ leukocytes were detectable in recipients by flow cytometry above the background of the assay (approximately 0.02% of total leukocytes).
The serum of naïve mice defined the background of the assay, and HSV-2 MS latently infected mice served as a positive control group that should possess bona fide immunity to secondary HSV-2 infection (Fig. 6A).
However, because these changes in controls were small in magnitude and were not always observed, we interpret these changes as being within the background of the assay.
Values from negative control leaves were used as "background" of the assay and were subtracted from the corresponding values obtained from DIII construct-infiltrated leaves.
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The magnitude of all sub-dominant responses was low, close to the value of background for the assay.
The background of this assay variant is significantly lower, supporting our explanation of the high background in assay variants 1 and 2. Furthermore, the novel assay has fewer steps and requires less time.
To calculate the viability of the brain slices (as a percentage) after the 5 h incubation-equilibration period, the following steps are taken for each experimental setup: Background absorbance control - provides information about the background absorbance of the assay medium (aECF).
In our analyses, any signal corresponding to incorrect bases was therefore considered to be the background noise of the assay.
As expected, serum IFN-γ concentration was undetectable (background level of the assay) in SEB challenged HLA-DR3.IFN-γ−/− mice whereas it ranged from 700 to 4000 pg/ml (mean 1830±387 pg/ml) in SEB challenged HLA-DR3.IFN-γ+/+ mice.
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background of the trials
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